Three T‐cell epitopes within the C‐terminal 265 amino acids of the matrix protein pp65 of human cytomegalovirus recognized by human lymphocytes
Identifieur interne : 001483 ( Istex/Checkpoint ); précédent : 001482; suivant : 001484Three T‐cell epitopes within the C‐terminal 265 amino acids of the matrix protein pp65 of human cytomegalovirus recognized by human lymphocytes
Auteurs : Barbara Anna-Maria Khattab [Allemagne] ; Werner Lindenmaier [Allemagne] ; Ronald Frank [Allemagne] ; Hartmut Link [Allemagne]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 1997-05.
English descriptors
- Teeft :
- Allele, Amino, Amino acids, Anchor motifs, Bone marrow transplantation, Cell response, Comparative analysis, Cytomegalovirus, Cytomegalovirus infection, Cytotoxic, Donor, Epitope, Fine mapping, Fusion protein responders, Fusion proteins, Hcmv, Hcmv antigen, Human cytomegalovirus, Immunology, Khattab, Lymphocyte, Lymphocyte proliferation, Lymphocyte proliferative responses, Matrix, Matrix protein, Medical school hannover, Medical virology, Monoclonal antibodies, Pbmc, Pentaplicate wells, Peptide, Proliferative, Proliferative responses, Recombinant, Responder, Riddell, Secondary antigens, Stimulation indices, Synthetic peptides, Virology.
Abstract
Although a T‐cell response in human cytomegalovirus (HCMV)‐immune individuals exists against the most abundantly expressed protein pp65 of the virus matrix, less is known about the determinants that evoke this response. The aim of the study was to identify regions within HCMV pp65 (ppUL83) that contain sequences for the cellular immune response by the use of three recombinant overlapping β‐galactosidase pp65 fusion proteins (C74, C35, and C47), covering the C‐terminal 265 amino acids of the entire pp65 sequence. Two T‐cell epitope determinants were recognized by human lymphocytes of healthy, HCMV‐seropositive, human leukocyte antigen (HLA)‐typed individuals. One T‐cell determinant (amino acids [aa] 303–388) was localized in the mid‐region of the entire pp65 sequence and a second T‐cell determinant (aa 477–561) within the C‐terminal region. By fine mapping with synthetic hexadecamer peptides three T‐cell epitopes were identified within these two regions: P10‐I (aa 361–376) in the mid‐region, P3‐II (aa) 485–499), and P6‐II (aa 509–524) in the C‐terminal region. Inhibition studies with monoclonal antibodies to HLA class I or class II revealed a class I restricted response to peptides P10‐I or P6‐II, respectively. P10‐I responders shared the HLA‐DR11 allele and P6‐II responders the ‐DR3 allele. Therefore, these T‐cell epitopes of HCMV pp65 might be presented in association with particular HLA class II alleles. J. Med. Virol. 52:68–76, 1997. © 1997 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/(SICI)1096-9071(199705)52:1<68::AID-JMV11>3.0.CO;2-X
Affiliations:
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last="Khattab">Barbara Anna-Maria Khattab</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Hematology and Oncology, Medical School, Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr" wicri:curation="lc">Allemagne</country><wicri:regionArea>Correspondence address: Department of Hematology and Oncology, OE 6860, Haus E, Medical School Hannover, 30623 Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Lindenmaier, Werner" sort="Lindenmaier, Werner" uniqKey="Lindenmaier W" first="Werner" last="Lindenmaier">Werner Lindenmaier</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Applied Genetics and Molecular Recognition, Gessellschaft für Biotechnologische Forschung mbH, Braunschweig</wicri:regionArea><wicri:noRegion>Braunschweig</wicri:noRegion><wicri:noRegion>Braunschweig</wicri:noRegion></affiliation></author><author><name sortKey="Frank, Ronald" sort="Frank, Ronald" uniqKey="Frank R" first="Ronald" last="Frank">Ronald Frank</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Applied Genetics and Molecular Recognition, Gessellschaft für Biotechnologische Forschung mbH, Braunschweig</wicri:regionArea><wicri:noRegion>Braunschweig</wicri:noRegion><wicri:noRegion>Braunschweig</wicri:noRegion></affiliation></author><author><name sortKey="Link, Hartmut" sort="Link, Hartmut" uniqKey="Link H" first="Hartmut" last="Link">Hartmut Link</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Hematology and Oncology, Medical School, Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">52</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="68">68</biblScope><biblScope unit="page" to="76">76</biblScope><biblScope unit="page-count">9</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="1997-05">1997-05</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Allele</term><term>Amino</term><term>Amino acids</term><term>Anchor motifs</term><term>Bone marrow transplantation</term><term>Cell response</term><term>Comparative analysis</term><term>Cytomegalovirus</term><term>Cytomegalovirus infection</term><term>Cytotoxic</term><term>Donor</term><term>Epitope</term><term>Fine mapping</term><term>Fusion protein responders</term><term>Fusion proteins</term><term>Hcmv</term><term>Hcmv antigen</term><term>Human cytomegalovirus</term><term>Immunology</term><term>Khattab</term><term>Lymphocyte</term><term>Lymphocyte proliferation</term><term>Lymphocyte proliferative responses</term><term>Matrix</term><term>Matrix protein</term><term>Medical school hannover</term><term>Medical virology</term><term>Monoclonal antibodies</term><term>Pbmc</term><term>Pentaplicate wells</term><term>Peptide</term><term>Proliferative</term><term>Proliferative responses</term><term>Recombinant</term><term>Responder</term><term>Riddell</term><term>Secondary antigens</term><term>Stimulation indices</term><term>Synthetic peptides</term><term>Virology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although a T‐cell response in human cytomegalovirus (HCMV)‐immune individuals exists against the most abundantly expressed protein pp65 of the virus matrix, less is known about the determinants that evoke this response. The aim of the study was to identify regions within HCMV pp65 (ppUL83) that contain sequences for the cellular immune response by the use of three recombinant overlapping β‐galactosidase pp65 fusion proteins (C74, C35, and C47), covering the C‐terminal 265 amino acids of the entire pp65 sequence. Two T‐cell epitope determinants were recognized by human lymphocytes of healthy, HCMV‐seropositive, human leukocyte antigen (HLA)‐typed individuals. One T‐cell determinant (amino acids [aa] 303–388) was localized in the mid‐region of the entire pp65 sequence and a second T‐cell determinant (aa 477–561) within the C‐terminal region. By fine mapping with synthetic hexadecamer peptides three T‐cell epitopes were identified within these two regions: P10‐I (aa 361–376) in the mid‐region, P3‐II (aa) 485–499), and P6‐II (aa 509–524) in the C‐terminal region. Inhibition studies with monoclonal antibodies to HLA class I or class II revealed a class I restricted response to peptides P10‐I or P6‐II, respectively. P10‐I responders shared the HLA‐DR11 allele and P6‐II responders the ‐DR3 allele. Therefore, these T‐cell epitopes of HCMV pp65 might be presented in association with particular HLA class II alleles. J. Med. Virol. 52:68–76, 1997. © 1997 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Basse-Saxe</li></region><settlement><li>Hanovre</li></settlement></list><tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Khattab, Barbara Anna Aria" sort="Khattab, Barbara Anna Aria" uniqKey="Khattab B" first="Barbara Anna-Maria" last="Khattab">Barbara Anna-Maria Khattab</name></region><name sortKey="Frank, Ronald" sort="Frank, Ronald" uniqKey="Frank R" first="Ronald" last="Frank">Ronald Frank</name><name sortKey="Khattab, Barbara Anna Aria" sort="Khattab, Barbara Anna Aria" uniqKey="Khattab B" first="Barbara Anna-Maria" last="Khattab">Barbara Anna-Maria Khattab</name><name sortKey="Lindenmaier, Werner" sort="Lindenmaier, Werner" uniqKey="Lindenmaier W" first="Werner" last="Lindenmaier">Werner Lindenmaier</name><name sortKey="Link, Hartmut" sort="Link, Hartmut" uniqKey="Link H" first="Hartmut" last="Link">Hartmut Link</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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